BI1BAB2-Metabolic and Practical Biochemistry

Module Provider: School of Biological Sciences
Number of credits: 20 [10 ECTS credits]
Level:4
Terms in which taught: Spring / Summer term module
Pre-requisites:
Non-modular pre-requisites: A Level Biology and GCSE Maths
Co-requisites:
Modules excluded: BI1MB2 Metabolic Biochemistry
Current from: 2021/2

Module Convenor: Dr Alice Pollitt
Email: a.pollitt@reading.ac.uk

Type of module:

Summary module description:

The regulation of metabolism is fundamental to life.Ìý From energy generation to the formation and breakdown of the building blocks which make up the human body (proteins, lipids and polysaccharides) these cellular metabolic processes are highly controlled and coordinated.Ìý Supported by practical sessions this module will explore the critical principles that underpin metabolism and how these are studied in a laboratory setting.

Aims:

This module aims to provide the student with an understanding of key biochemical concepts such as enzyme kinetics, metabolic pathways (carbohydrate, protein and lipids), metabolic regulation, and pathological metabolism (including inherited metabolic diseases). The associated spring practical work aims to give students an opportunity to further investigate and reinforce knowledge of key lecture biochemical topics through practical sessions. The module will, therefore, give students experience and training in experimental projects, which form the basis of final year projects and real world scientific research. Further aims include: - To provide students an opportunity to plan and perform their own experiments; - To provide an illustration of the link between experimentation and scientific understanding; - To provide students with experience in scientific calculations, laboratory report writing and the analytical skills required to interpret experimental data.

Assessable learning outcomes:

By the end of the module it is expected that the student will be able to: • Describe basic metabolic pathways, anabolic and catabolic • Use enzyme kinetics to evaluate enzyme activities and their regulation • Describe the principles of metabolic regulationÌý• Understand principles of key lab methods • Record, analyse and interpret experimental data • Present and describe experimental results, critically analyse experimentsÌý

Additional outcomes:
Students should obtain a deeper understanding of biochemistry though the application of practical approaches and will have the opportunity to work as part of a team. Additionally, the mini-project aspect of the module will allow students to gain experience in problem solving and managing their own lab work.

Outline content:

This module will provide a fundamental understanding of metabolic processes common in cellular organisms. The operation of key pathways will be considered, the function of enzymes will be addressed and the manner in which biochemical processes are controlled will be highlighted. The module is designed to provide essential background on biochemical metabolic pathways that will enable students to comprehend biochemistry-related modules encountered later in their course. Students will engage in a series of practicals that will introduce essential biochemistry techniques and will illustrate key biochemical principles that will be of value in subsequent modules. Students will then apply the principles they have learnt to aÌýpractical mini-project. Students will also be introduced to ‘good laboratory practice’ (GLP), which they will be expected to follow throughout the practical sessions. There will be approximately 40 hours of practicals with calculation, analysis, writin g and planning sessions provided throughout the course to support training of students in these areas. The Lecture Content covers: • Basic enzyme kinetics • Carbohydrate and fatty acidÌýmetabolismÌý• Lipoproteins and cholesterol metabolism • Integration of metabolism – how pathways interact, control of the TCA cycle and oxidative phosphorylation, disturbed metabolism in starvation Ìý• Inborn errors of metabolism The Practical Content covers: • Spec troscopy • Western blotting • Chromatography • ELISA • Enzyme kinetics • Protein concentration determination • Protein purification • Tuition/guidance for calculations and write-ups

Global context:

Brief description of